There are many different ways to make biopsy specimens, which can be divided into two categories: non-slicing method and slicing method: non-slicing method, such as smear, paving, tableting, grinding and assembling; slicing method includes paraffin Slice method, collodion slice method, frozen section method.
1, non-slice method
That is, the method of slicing without slicing machine and without slicing procedure, there are different treatment methods according to the nature of the material, the method is simple and quick to operate, in which the laying method and the occluding method can make the original organizational structure not Destruction, smear, tableting method to make up for the use of embedding, slicing method can not be clearly observed deficiencies, it is commonly used in the production of microscopic specimens.
1) smear method
Mainly used for blood, semen, urine, sputum, microorganisms and other liquid can not be sliced granular material can be coated on glass slides into monolayer cells, and then fixed, dehydration, staining and other means to make a permanent specimen.
2) laying method
Mainly used for dynamic and plant tissue epidermal observation, living animals can be taken to observe animals and plants, tweezers with a torn layer of epidermis, quickly tiled on the slide. Such as the preparation of onion epidermal cells.
3) tabletting method
Some of the more tender, soft material can be placed on glass slides, with a small scalpel to disperse it, add a drop of dye, and then covered with cover glass, with a thumb vertical force squeeze, the tissue scattered into a thin, and then Observation, such as plant root tip observation of chromosomes, pollen grains observed developmental stages.
4) Isolation method
The method is to use chemicals to dissolve the interstitial cells of the tissue and to disperse the cells into individual individuals. After staining, dehydration, transparent to observe the individual morphology, suitable for muscle, leaves, stems and other parts.
5) grinding film
For very hard tissues such as bone and teeth.
2, slice method
Slice method is to rely on hand or slicer tissue cut into thin slices for observation. In order to clearly observe the structure and cell morphology of animals and plants, we must first go through a series of steps to infiltrate into the tissue some of the support material to harden the tissue to facilitate slicing. Depending on the type of support used, Divided into free-hand slicing method, paraffin section method, collodion cut method, frozen slice method and other types, after slicing also need to go wax, dyeing, dehydration, transparent and other steps, made of permanent specimens.
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