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The correct use of the microscope
- Jan 22, 2018 -

First, the correct installation of the problem

Before using a microscope, the eyepiece and objective of the microscope should be installed first. Eyepiece installation is very simple, the main problem is the installation of objective lens, because the objective lens is more expensive, if the student installation thread is not good, easy to fall to the ground, resulting in damage to the lens, so to be on the safe side, emphasizing the students to install the lens Left index finger with the middle finger holding the objective lens, and then use the right hand to mount the objective, so that even if not installed, it will not fall to the ground.

Second, the correct light on the issue

The light is a very important step when using the microscope, some students in the light, just turn an objective lens through the light hole, rather than by the low power of the mirror on the light. Turn the mirror like to use one hand, often pull the mirror down. Therefore, teachers in the guidance of students, we must emphasize the use of low power mirror on the light, when the light is strong with a small aperture, flat mirror, and the light is weak with a large aperture, concave mirror, mirror to rotate with both hands, when looking To a uniform bright circular view so far. After the light is right, do not move the microscope casually so as to prevent the light from entering the light hole accurately through the reflector.

Third, the correct use of quasi-coke spiral problem

Using a quasi-coke to adjust the focal length, find the object can be said that the microscope is the most important step in use, but also students feel the most difficult step. Students prone to the following errors in the operation: First, direct focus at high magnification; Second, regardless of the tube up or down, the eye is always in the eyepiece field of vision; Third, do not understand the object distance threshold, To 2 to 3 cm when still up, and the rotation of the quasi-coke spiral quickly. The first two errors often result in lens misalignment, damage to the lens or lens attachment, and the third error is one of the most common phenomena students use when using a microscope. For the above error, teachers must emphasize to students, adjust the focal length must be reduced at low magnification, turn the coarse coke first, so that the lens tube slowly down, the objective lens close to the slide, but be careful not to touch the objective lens In this process, the eye should look at the objective lens from the side, and then use the left eye to gaze into the eyepiece and slowly reverse the coarse coke lens so as to gradually raise the lens barrel until the image is seen. At the same time, General microscope object distance of about 1 cm, so if the object distance has been far more than 1 cm, but still do not see the object, it may be the specimen is not in sight or turn coarse quasi-focus spiral too fast, this time should be adjusted Loading film position, and then repeat the above steps, when the blurred vision appears in the field of vision, it is necessary to switch to a fine coke spiral adjustment, the only way to narrow the search range, improve the speed of finding objects.

Fourth, the problem of objective conversion

The use of low magnification lens for high magnification, students often like to directly push the objective lens with your fingers, that is more labor-saving, but it is easy to deflect the optical axis of the objective lens, because the converter material texture is soft, high accuracy , Uneven thread stress is easy to loose. Once the thread breaks, the entire converter will be scrapped. The teacher should instruct the student to hold the converter in the lower level to turn the conversion lens.

Fifth, optical glass cleaning problems

Optical glass used in instrument lenses, prisms, lenses and so on. In the manufacture and use of easy to get stained with oil, water-wet dirt, fingerprints, affecting imaging and transmittance. Cleaning optical glass, should be based on the characteristics of dirt, different structures, use different cleaning agents, use different cleaning tools, use different cleaning methods. Clean with AR coated lenses, such as cameras, slide projector, microscope lens, can be about 20% alcohol and about 80% of the ether configuration cleaning agent for cleaning. Apply a soft brush or cotton ball stained with a small amount of cleaning agent to do circular motion outward from the center of the lens. Avoid immersion in such cleaning agents cleaning lenses, cleaning lenses do not force wipe, or it will damage the AR, damage to the lens.